质粒名称: | pLenti CMV/TO p16 Hygro (w184-1) |
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出品公司: | 爱迪基因 |
目录编号: | 22263 |
存储实验室: | Eric Campeau |
载体骨架基本信息 |
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载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | 8722 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳动物表达,慢病毒 |
筛选标记: | 潮霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | cyclin-dependent kinase inhibitor 2A,p16 |
物种来源: | H. sapiens (human) |
插入大小: | 446 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | Bam HI (未破坏) |
3' 克隆位点: | Xba I (未破坏) |
5' 测序引物 | CMVforw |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pLenti CMV/TO p16 Hygro (w184-1) was a gift from Eric Campeau (Addgene plasmid # 22263 ; http://n2t.net/addgene:22263 ; RRID:Addgene_22263) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLenti CMV/TO p16 Hygro (w184-1) 9168 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLenti CMV/TO p16 Hygro (w184-1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9168) TITLE shMDC1 plasmids REFERENCE 2 (bases 1 to 9168) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9168 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 27..131 /gene="bla" /label=AmpR promoter CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(350..369) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1652..1671 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1905..1922 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2039..2060 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2075..2105 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2113..2129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2118..2140 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2137..2153 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2137..2153 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2172..2192 /label=T3 /note="T3 promoter, forward primer" promoter 2174..2192 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" LTR 2448..2628 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2672..2797 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3290..3523 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3707..3751 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4012..4129 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 4152..4455 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4456..4659 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4610..4630 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 4661..4679 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 4682..4700 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 4741..4765 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(5293..5317) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 5348..5936 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5401..5421) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5819..5830) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 5992..6491 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(6027..6050) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 6424..6443 /label=mPGK-F /note="Mouse PGK promoter, forward primer" LTR 7909..8142 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8214..8335 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8251..8270) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8305..8324 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8354..8489 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8416..8435 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(8521..8540) /label=T7 /note="T7 promoter, forward primer" promoter complement(8522..8540) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8550..8567) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8550..8566) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8559..8581) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 8711..9166 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8798..8817) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9008..9029 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct 4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 4321 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 4381 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 4441 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 4501 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 4561 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 4621 ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat 4681 ctccctatca gtgatagaga tcgtcgacta gtccagtgtg gtggaattct gcagatatca 4741 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatccagcat 4801 ggagccttcg gctgactggc tggccacggc cgcggcccgg ggtcgggtag aggaggtgcg 4861 ggcgctgctg gaggcggggg cgctgcccaa cgcaccgaat agttacggtc ggaggccgat 4921 ccaggtcatg atgatgggca gcgcccgagt ggcggagctg ctgctgctcc acggcgcgga 4981 gcccaactgc gccgaccccg ccactctcac ccgacccgtg cacgacgctg cccgggaggg 5041 cttcctggac acgctggtgg tgctgcaccg ggccggggcg cggctggacg tgcgcgatgc 5101 ctggggccgt ctgcccgtgg acctggctga ggagctgggc catcgcgatg tcgcacggta 5161 cctgcgcgcg gctgcggggg gcaccagagg cagtaaccat gcccgcatag atgccgcgga 5221 aggtccctca gacatccccg attgaaagga attctgcaga tatccatcac actggccgct 5281 cgagatatct agacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc 5341 gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 5401 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 5461 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 5521 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 5581 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 5641 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 5701 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 5761 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 5821 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 5881 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 5941 aattctgcag atatccggac tagtgatctc tcgaggttaa cgaattctac cgggtagggg 6001 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc 6061 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg 6121 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc 6181 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta 6241 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg 6301 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg 6361 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc 6421 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc 6481 cgggcctttc gacctgcatc ccgccaccat gaaaaagcct gaactcaccg cgacgtctgt 6541 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg 6601 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa 6661 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc 6721 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat 6781 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt 6841 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag 6901 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat 6961 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag 7021 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt 7081 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat 7141 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa 7201 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg 7261 gaggcatccg gagcttgcag gatcgccgcg gctccggggc gtatatgctc cgcattggtc 7321 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg 7381 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc 7441 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc 7501 gacgccccag cactcgtccg agggcaaagg aatagagtag atgccgaccg aacaagagct 7561 gatttcgaga acgcctcagc cagcaactcg cgcgagccta gcaaggcaaa tgcgagagaa 7621 cggccttacg cttggtggca cagttctcgt ccacagttcg ctaagctcgc tcggctgggt 7681 cgcgggaggg ccggtcgcag tgattcaggc ccttctggat tgtgttggtc cccagggcac 7741 gattgtcatg cccacgcact cgggtgatct gactgatccc gcagattgga gatcgccgcc 7801 cgtgcctgcc gattgggtgc agatccgtcg agggcccggt acctttaaga ccaatgactt 7861 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag 7921 ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 7981 agatctgagc ctgggagctc tctggctgcc tagggaaccc actgcttaag cctcaataaa 8041 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 8101 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 8161 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 8221 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 8281 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 8341 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 8401 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 8461 gaagtagtga ggaggctttt ttggaggcct aggcttttgc gtcgagacgt acccaattcg 8521 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg 8581 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 8641 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 8701 gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 8761 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 8821 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 8881 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 8941 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 9001 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 9061 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 9121 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcc //
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